Rabbit Anti-PEA3/FITC Conjugated antibody

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Adenovirus E1A enhancer binding protein; Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein E1AF); ETV4; ETV4_HUMAN; PEA3; PEA3 protein; PEAS3; Polyomavirus enhancer activat

Cat:

SL1869R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Pig,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human PEA3

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

53kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAASLC3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

Function:
Pea3 is a member of the Ets gene family which are known to encode sequence specific DNA binding proteins. Pea3 binds the motif 5-AGGAAG-3 (the Pea-3 motif). It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. Pea3 is detected in cells of epithelial and fibroblastic origin. It is also an transcriptional activator that binds to the enhancer of the adenovirus E1A gene.

Subcellular Location:
Nucleus.

Post-translational modifications:
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.

Similarity:
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.

Database links:

Entrez Gene: 2118 Human

Entrez Gene: 18612 Mouse

Omim: 600711 Human

SwissProt: P43268 Human

SwissProt: P28322 Mouse

Unigene: 434059 Human

Unigene: 5025 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PEA3属癌基因的转录因子Ets家族成员之一,多定位于细胞核。多瘤促活化因子(polymoma virus enhancer-3,PEA3)广泛表达于小鼠神经系统中,现有的研究已证实,PEA3与神经细胞的迁移、分化以及神经突触的形成过程有关.

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