Rabbit Anti-CX3CR1/FITC Conjugated antibody

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Beta chemokine receptor-like 1; C X3 C CKR 1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMKBLR1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; Fractalkine Receptor; GPR13; GPRV28; V28; Chemokine receptor 1(chemokine (C-X3-C motif) r

Cat:

SL1728R-FITC

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CX3CR1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=2ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

39kDa

Product Overview：

Blank control:U937. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.Blank control:U251. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HISLV1, and some variations in this gene lead to increased susceptibility to HISLV1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Function:
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HISLV1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HISLV1 coreceptors than isoform 1.

Subunit:
Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response. Interacts with HISLV1 envelope polyprotein gp160.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in lymphoid and neural tissues.

DISEASE:
Age-related macular degeneration 12 (ARMD12) [MIM:613784]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Database links:

Entrez Gene: 1524 Human

Entrez Gene: 13051 Mouse

Entrez Gene: 171056 Rat

Omim: 601470 Human

SwissProt: P49238 Human

SwissProt: Q9Z0D9 Mouse

SwissProt: P35411 Rat

Unigene: 78913 Human

Unigene: 8865 Mouse

Unigene: 10482 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:U251.
Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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