Growth Arrest And DNA Damage Inducible Alpha;
DNA Damage-Inducible Transcript 1 Protein;
DDIT-1;
GADD45;
DDIT1;
Growth Arrest And DNA Damage-Inducible Protein GADD45 Alpha;
Growth Arrest And DNA-Damage-Inducible 45 Alpha;
Growth Arrest And DNA-Dama
Cat:
SL172R-FITC
Species Reactivity：
Human,Mouse,Rat,(predicted: Chicken,Pig,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human GADD45
Format：
Lyophilized or Liquid
Storage instructions：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer：
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
Flow-Cyt=1:50-200ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Calculated MW：
18kDa
Product Overview：
Blank control: A431. Primary Antibody (green line): Rabbit Anti-GADD45A antibody (SL172R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53 dependent and independent mechanisms.
Function: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
Subunit: Interacts with MAPK14. Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization.
Subcellular Location: Nucleus.
Similarity: Belongs to the GADD45 family.
Database links:
Entrez Gene: 1647 Human
Entrez Gene: 13197 Mouse
Entrez Gene: 25112 Rat
Omim: 126335 Human
SwissProt: P24522 Human
SwissProt: P48316 Mouse
SwissProt: P48317 Rat
Unigene: 80409 Human
Unigene: 72235 Mouse
Unigene: 10250 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
GADD45α蛋白与细胞凋亡和死亡密切相关,在某种程度上决定了细胞周期的进程。
Picture
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-GADD45A antibody (SL172R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
