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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti- GDNF Antibody, conjugated (SL1024R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti- GDNF Antibody, conjugated (SL1024R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.
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