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Rabbit Anti-APAF1(CT)/FITC Conjugated antibody
background:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain(NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Function:
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Subunit:
Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain).
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Similarity:
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
Database links:
Entrez Gene: 317 Human
Entrez Gene: 11783 Mouse
Entrez Gene: 78963 Rat
Omim: 602233 Human
SwissProt: O14727 Human
SwissProt: O88879 Mouse
SwissProt: Q9EPV5 Rat
Unigene: 728891 Human
Unigene: 220289 Mouse
Unigene: 64522 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Apaf1 有称:凋亡酶激活因子-1(Apoptosis protease activating factor-1)调节细胞色素C依赖的Caspase-9原的自动催化活性,导致Caspase-3 的激活和引起凋亡。
Apaf-1在成年人的脾脏、外周血白细胞、肾脏、肺和胎儿脑、肾、肺中高水平表达。
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