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Rabbit Anti-HIC5/FITC Conjugated antibody
background:
Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16.
Function:
Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
Subunit:
Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7 (By similarity). Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin.
Subcellular Location:
Cell junction; focal adhesion. Nucleus matrix. Cytoplasm; cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
Tissue Specificity:
Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
Post-translational modifications:
Phosphorylated by gonadotropin-releasing hormone-activated SRC.
Similarity:
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Database links:
Entrez Gene: 7041 Human
Entrez Gene: 2364 Mouse
Entrez Gene: 84574 Rat
Entrez Gene: 515834 Cow
Omim: 602353 Human
SwissProt: Q3MHZ4 Cow
SwissProt: O43294 Human
SwissProt: Q9BPW3 Human
SwissProt: Q3YBY7 Mouse
SwissProt: Q3YBY8 Mouse
SwissProt: Q3YBZ0 Mouse
SwissProt: Q3YBZ1 Mouse
SwissProt: Q3YBZ3 Mouse
SwissProt: Q3YBZ4 Mouse
SwissProt: Q3YBZ5 Mouse
SwissProt: Q3YBZ6 Mouse
SwissProt: Q62219 Mouse
SwissProt: Q99PD6 Rat
Unigene: 513530 Human
Unigene: 3248 Mouse
Unigene: 103260 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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