background:
This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012].
Function:
Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Subunit:
Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB.
Subcellular Location:
Cytoplasm. Nucleus. Lysosome. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
Similarity:
Belongs to the GTR/RAG GTP-binding protein family.
Database links:
Entrez Gene: 64121 Human
Entrez Gene: 54170 Mouse
Entrez Gene: 100528436 Pig
Entrez Gene: 298514 Rat
Omim: 608267 Human
SwissProt: Q9HB90 Human
SwissProt: Q99K70 Mouse
Unigene: 532461 Human
Unigene: 220922 Mouse
Unigene: 473825 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-RRAGC antibody (SL10097R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): Mouse spleen.
Primary Antibody (green line): Rabbit Anti-RRAGC antibody (SL10097R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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