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Rabbit Anti-Drosha/FITC Conjugated antibody
background:
The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.
Function:
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Subunit:
Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with DGCR8, SP1 and SNIP1. Interacts with SRRT/ARS2.
Subcellular Location:
Nucleus. Nucleus; nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
Tissue Specificity:
Ubiquitous.
Similarity:
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains.
Database links:
Entrez Gene: 29102 Human
Entrez Gene: 1800 Mouse
Omim: 608828 Human
SwissProt: Q9NRR4 Human
SwissProt: Q5HZJ0 Mouse
Unigene: 97997 Human
Unigene: 293142 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Dircer和Drosha是两种核糖核酸酶是卵巢癌细胞中RNA干扰机制中的关键酶,Dircer和Drosha两种酶的表达(数量)越高,存活时间越长。美国科学家研究发现,卵巢癌患者存活率与两种酶有关。美国MD安德森癌症治疗中心先后对111个和132个卵巢癌样本进行了分析。结果发现,人体内两种核糖核酸酶Dicer和Drosha含量高的患者平均存活11年,而含量低的患者平均只能活2.86年。Dicer的水平高低对卵巢癌患者尤其重要。这一发现有助于卵巢癌的诊断和治疗。卵巢恶性肿瘤的发病率在女性常见恶性肿瘤中占2.4%~5.6%,在女性生殖道恶性肿瘤中占第三位。在妇女生殖道癌瘤中,卵巢癌造成的死亡人数高居首位。
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