Rabbit Anti-ENT1/FITC Conjugated antibody

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Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside tr

Cat:

SL13077R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Cow,Horse,Sheep,Cynomolgus Monkey, Orangutan)

Immunogen：

KLH conjugated synthetic peptide derived from human ENT1/SLC29A1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

50kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Equilibrative nucleoside transporters (ENTs) regulate many physiological processes and are widely distributed in mammals, plants, yeasts, insects, nematodes and protozoans. They enable facilitated diffusion of hydrophilic nucleosides, such as adenosine and nucleoside analogs, across cell membranes. ENTs are required for uptake of antiviral and anticancer nucleoside drugs and influence a variety of physiological processes, such as neurotransmission and platelet aggregation, by regulating the amount of adenoside available to cell surface receptors. Equilibrative nucleoside transporter 1 (ENT1), also designated solute carrier family 29 (nucleoside transporters), member 1, belongs to the SLC29A transporter family and is a mammalian ENT isoform. ENT1, along with ENT3, mediates the majority of influx and efflux of nucleosides across the plasma membrane.

Function:
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Subcellular Location:
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.

Tissue Specificity:
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Post-translational modifications:
Glycosylated.

Similarity:
Belongs to the SLC29A transporter family.

Database links:

Entrez Gene: 2030 Human

Entrez Gene: 63959 Mouse

Omim: 602193 Human

SwissProt: Q91968 Human

SwissProt: Q9JIM1 Mouse

Unigene: 25450 Human

Unigene: 29744 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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