Rabbit Anti-Supervillin/FITC Conjugated antibody

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Archvillin; p205/p250; SVIL_HUMAN.

Cat:

SL7123R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Supervillin

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

248kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Supervillin is an F-actin-binding protein originally with two reported isoforms of 205/250 kDa. The longer isoform, named archvillin, is muscle-specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. It contributes to cell-cell adhesion, motility regulation, and information transfer between cell compartments. The COOH-terminus of supervillin is homologous to villin/gelsolin but is not responsible for the tight binding to the actin cytoskeleton in vivo. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland, and salivary gland.

Function:
Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adehesions involves binding to TRIP6 (By similarity).

Subunit:
Associates with F-actin. Interacts with NEB. Interacts with MYH9. Interacts with MYLK. Isoform 2 interacts with TRIP6. Isoform 2 interacts with DYNLT1 (By similarity).

Subcellular Location:
Cell membrane; peripheral membrane protein; cytoplasmic side. Cytoplasm; cytoskeleton.

Tissue Specificity:
Expressed in many tissues. Most abundant in muscle, bone marrow, thyroid gland and salivary gland. Isoform 1 (archvillin) is muscle specific.

Similarity:
Belongs to the villin/gelsolin family.
Contains 5 gelsolin-like repeats.
Contains 1 HP (headpiece) domain.

Database links:

Entrez Gene: 6168 Human

Omim: 604126 Human

SwissProt: O95425 Human

SwissProt: Q569J5 Human

Unigene: 499209 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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