Rabbit Anti-C3IP1 /FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-C3IP1 /FITC Conjugated antibody

C3IP1; CUL3 interacting protein 1; CUL3-interacting protein 1; DKIR; FLJ27152; Kelch like 12 (Drosophila); Kelch like 12; Kelch like 12 variant; Kelch like protein 12; kelch like protein C3IP1; Kelch-like protein 12; KLH12_HUMAN; KLHL 12; KLHL12; OTTHUMP0

Cat:

SL15164R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human C3IP1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

63kDa

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
KLHL12 is a 568 amino acid protein that is a component of an ubiquitin-protein E3 ligase complex, which includes at least CUL-3. KLHL12 is a substrate-specific adapter for the complex, which negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of Dvl-3. KLHL12 contains six Kelch repeats and one BTB (POZ) domain, which is required for interaction with CUL-3. KLHL12 has highest expression in testis, with lower levels found in the submandibular salivary gland. The gene that encodes KLHL12 maps to human chromosome 1q32.1

Function:
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.

Subunit:
Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A.

Subcellular Location:
Cytoplasmic vesicle, COPII-coated vesicle.

Tissue Specificity:
Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.

Similarity:
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

Database links:

Entrez Gene: 713668 Cow

Entrez Gene: 59349 Human

Entrez Gene: 48756 Mouse

Entrez Gene: 266772 Rat

Entrez Gene: 431860 Xenopus laevis

Entrez Gene: 492362 Zebrafish

Omim: 614522 Human

SwissProt: E1B932 Cow

SwissProt: Q53G59 Human

SwissProt: Q8BZM0 Mouse

SwissProt: Q8R2H4 Rat

SwissProt: Q6NRH0 Xenopus laevis