Rabbit Anti-GEM/FITC Conjugated antibody

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GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR;

Cat:

SL13331R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human GEM

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

34kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical SLCterminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this SLCterminal extension, involving residues 278–297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome 8q22.1.

Function:
GEM belongs to the RAD/GEM family of GTP-binding proteins. It could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. GEM has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Subunit:
Interacts with calmodulin in a Ca(2+)-dependent manner. Binds ROCK1.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Belongs to the small GTPase superfamily. RGK family.

Database links:

Entrez Gene: 2669 Human

Entrez Gene: 14579 Mouse

Entrez Gene: 297902 Rat

Omim: 600164 Human

SwissProt: P55040 Human

SwissProt: P55041 Mouse

Unigene: 654463 Human

Unigene: 247486 Mouse

Unigene: 40436 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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