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Rabbit Anti-KDM1/LSD1/FITC Conjugated antibody
background:
Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Lysine specific demethylase 1 (KDM1/LSD1) is a long-sought histone demethylase that specifically demethylates mono and di methyl histone H3 at K4 and K9. Thus KDM1 is a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
Function:
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
Subunit:
Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B (By similarity). Component of a SHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The SHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/SHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Ubiquitously expressed.
Similarity:
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain.
Database links:
Entrez Gene: 23028 Human
Entrez Gene: 99982 Mouse
Entrez Gene: 500569 Rat
Omim: 609132 Human
SwissProt: O60341 Human
SwissProt: Q6ZQ88 Mouse
Unigene: 591518 Human
Unigene: 28540 Mouse
Unigene: 203461 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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