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Rabbit Anti-HECT E3 ubiquitin ligase/FITC Conjugated antibody
background:
HECT E3 ubiquitin ligase is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
Function:
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
Subunit:
Binds to CCNA1, CCNB1, CCND1 and CCNE1. Interacts with UBE2L6. Interacts with IRF3, this interaction is marginal in resting cells but enhanced upon viral infection.
Subcellular Location:
Cytoplasm, perinuclear region. Note=Associated with the polyribosomes, probably via the 60S subunit.
Tissue Specificity:
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
Post-translational modifications:
ISGylated (Probable).
Similarity:
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 5 RCC1 repeats.
Database links:
Entrez Gene: 51191 Human
Omim: 608242 Human
SwissProt: Q9UII4 Human
Unigene: 26663 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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