Rabbit Anti-ROCK1 + ROCK2/FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-ROCK1 + ROCK2/FITC Conjugated antibody

p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil c

Cat:

SL10173R-FITC

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ROCK1 and ROCK

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

161kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].

Function:
Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.

Subunit:
Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads toconstitutive activation of the kinase and membrane blebbing.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family.
Contains 1 AGSLCkinase SLCterminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.

Database links:

Entrez Gene: 6093 Human

Entrez Gene: 19877 Mouse

Entrez Gene: 81762 Rat

Omim: 601702 Human

SwissProt: Q13464 Human

SwissProt: P70335 Mouse

SwissProt: Q63644 Rat

Unigene: 306307 Human

Unigene: 6710 Mouse

Unigene: 89756 Rat

Entrez Gene: 9475 Human

Entrez Gene: 19878 Mouse