Rabbit Anti-phospho-NFATC4 (Ser168 + Ser170)/FITC Conjugated antibody

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NFATC4 (phospho S168 + S170); p-NFATC4 (phospho S168 + S170); NFATC4 cytoplasmic 4; NF ATc4; NF-AT3; NF-ATc4; NFAC4_HUMAN; NFAT3; NFATc4; Nuclear factor of activated T cells cytoplasmic 4; Nuclear factor of activated T cells cytoplasmic calcineurin depend

Cat:

SL8535R-FITC

Species Reactivity：

(predicted: Human,Mouse,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human NFATC4 around the phosphorylation site of Ser168 + Ser170

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

95kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The product of this gene is a member of the nuclearfactors of activated T cells DNA-binding transcription complex.This complex consists of at least two components: a preexistingcytosolic component that translocates to the nucleus upon T cellreceptor (TCR) stimulation and an inducible nuclear component.Other members of this family of nuclear factors of activated Tcells also participate in the formation of this complex. Theproduct of this gene plays a role in the inducible expression ofcytokine genes in T cells, especially in the induction of the IL-2and IL-4. Alternatively spliced transcript variants encodingdifferent isoforms have been noted for this gene. [provided byRefSeq, Oct 2008].

Function:
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.

Subunit:
Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

Tissue Specificity:
Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Post-translational modifications:
Phosphorylated by NFATSLCkinases; dephosphorylated bycalcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1,MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and onSer-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.

Similarity:
Contains 1 IPT/TIG domain.
Contains 1 RHD (Rel-like) domain.
Database links : UniProtKB/Swiss-Prot: Q14934.2

Database links:

Entrez Gene: 4776 Human

Entrez Gene: 73181 Mouse

Entrez Gene: 305897 Rat

Omim: 602699 Human

SwissProt: Q14934 Human

SwissProt: Q8K120 Mouse

Unigene: 77810 Human

Unigene: 27908 Mouse

Unigene: 23727 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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