Rabbit Anti-IFIT1 /FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-IFIT1 /FITC Conjugated antibody

C56; G10P1; GARG 16; GARG16; IFI 56; IFI 56K; IFI-56K; IFI56; IFIT-1; Ifit1; IFIT1_HUMAN; IFNAI1; Interferon alpha inducible protein (MW 56kD); Interferon induced 56 kDa protein; Interferon induced protein 56; Interferon inducible mRNA 561; Interferon-ind

Cat:

SL15553R-FITC

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human IFIT1

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

55kDa

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-IFIT1 Antibody, conjugated (SL15553R-FITC) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012]

Function:
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.

Subunit:
Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts (via TPR repeats 4-7) with EEF1A1 (By similarity). Interacts with EIF3C (By similarity). Interacts with IFIT2 and IFIT3. Interacts (via TPR repeats 1-4) with RPL15. Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E. Interacts (via TPR repeat 2) with E1 proteins of HPV types 11, 18 and 3 and this interaction blocks E1 helicase activity and viral replication.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylated.

Similarity:
Belongs to the IFIT family.
Contains 10 TPR repeats.

Database links:

Entrez Gene: 3434 Human

Omim: 147690 Human

SwissProt: P09914 Human

Unigene: 20315 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Product Feedback Wall