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Rabbit Anti-GRB 14/FITC Conjugated antibody
background:
Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phospho-tyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its SLCterminal, a central region with similarity to Ras GAP and a proline-rich N-terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a Pleckstrin homology domain in its central region and a carboxy-terminal SH2 domain. GRB14 mRNA is expressed at high levels in a broad range of tissues including liver, kidney, pancreas, testis, ovary, heart and skeletal muscle. Expression of the GRB14 protein in breast carcinomas is strongly correlated with estrogen receptor positivity.
Function:
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date.
Subunit:
Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor (INSR), through the SH2 domain (By similarity). Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation. Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).
Subcellular Location:
Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Endosome membrane.
Tissue Specificity:
Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
Post-translational modifications:
Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.
Similarity:
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
Database links:
Entrez Gene: 2888 Human
Entrez Gene: 50915 Mouse
Entrez Gene: 58844 Rat
Omim: 601524 Human
SwissProt: Q14449 Human
SwissProt: Q9JLM9 Mouse
SwissProt: O88900 Rat
Unigene: 411881 Human
Unigene: 214554 Mouse
Unigene: 30028 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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