Rabbit Anti-NSMAF/FITC Conjugated antibody

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Factor associated with N SMase activation; Factor associated with neutral sphingomyelinase; Factor associated with neutral sphingomyelinase activation; FAN; Neutral sphingomyelinase (N SMase) activation associated factor; Protein FAN.

Cat:

SL13656R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NSMAF

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

104kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1,2). These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types (1,3). The majority of TNF functions are primarily mediated through TNF-R1 (1,4). FAN (for factor associated with neutral sphingomyelinase (N-SMase) activation) is an intermediate protein that interacts with TNF-R1 to initiate TNF signaling events. FAN binds to TNF-R1 at the cytoplasmic NSD (N-SMase activating domain), which results in the initiation of the N-SMase pathway (5). N-SMase has been shown to be involved in TNF-induced Raf-1 activation (6). FAN contains four carboxy-terminal WD-repeat domains which appear to be involved in protein-protein interaction. The FAN WD-repeats may mediate the interaction between FAN and TNF-R1 (5).

Function:
NSMAF is a novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for reducing ischemia/reperfusion injury.

Tissue Specificity:
Ubiquitous.

Similarity:
Contains 1 BEACH domain.
Contains 1 GRAM domain.
Contains 6 WD repeats.

Database links:

Entrez Gene: 8439 Human

Entrez Gene: 18201 Mouse

Entrez Gene: 353233 Rat

Omim: 603043 Human

SwissProt: Q92636 Human

SwissProt: O35242 Mouse

SwissProt: Q7TSY5 Rat

Unigene: 314400 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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