Rabbit Anti-Chemokine Receptor D6/FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-Chemokine Receptor D6/FITC Conjugated antibody

C C chemokine receptor D6; C-C chemokine receptor D6; CC chemokine binding receptor JAB61; CC chemokine receptor D6; CCBP 2; CCBP2; ACKR2_HUMAN; CCR 10; CCR 9; CCR10; CCR9; Chemokine (C C motif) receptor 9; Chemokine (C C) receptor 9; Chemoki

Cat:

SL13897R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Pig,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Chemokine Receptor D6

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

43kDa

Product Overview：

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-Chemokine Receptor D6 antibody (SL13897R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Chemokine Receptor D6 is a member of the SLCC (β chemokine) G protein-coupled receptor family. This family is characterized by a pair of adjacent cysteine residues. SLCC chemokine receptor family members also include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10, CCXCKR, Bonzo, BOB (brother of Bonzo) and Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to sites of inflammation. Chemokine Receptor D6 acts as a receptor for various SLCC type chemokines, including SCYA2/MCP-1, SCY3/MIP-1-α, SCYA5/RANTES and SCYA7/MCP-3. It is expressed primarily in placental tissues and fetal liver, but is also detected in lymphatic endothelial cells, lymph nodes and mucosa of the small and large intestines.

Function:
Receptor for SLCC type chemokines including SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Database links:

Entrez Gene: 1238 Human

Omim: 602648 Human

SwissProt: O00590 Human

Unigene: 146346 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-Chemokine Receptor D6 antibody (SL13897R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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