Rabbit Anti-Cingulin/FITC Conjugated antibody

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6330408J11Rik; AI647528; AI987749; cgn; CING_HUMAN; Cingulin; DKFZp779N1112; FLJ39281; KIAA1319; MGC118157.

Cat:

SL13956R-FITC

Species Reactivity：

Human,(predicted: Mouse,Rat,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Cingulin

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

136kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Diffusion of solutes is prevented across certain barriers by the formation of tight junction seals. Occludin and Cingulin interact with other proteins to direct the formation and regulation of tight junctions. Cingulin, a protein component of the submembrane plaque of tight junctions (TJ), contains globular and coiled-coil domains and interacts in vitro with several TJ and cytoskeletal proteins, including the PDZ protein ZO-1. Cingulin binding has also been shown to inhibit RhoA activation and signaling with increased Cingulin expression in confluent cells, causing downregulation of RhoA by inhibiting GEF-H1/Lfc.

Function:
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular Location:
Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.

Tissue Specificity:
Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.

Similarity:
Belongs to the cingulin family.

Database links:

Entrez Gene: 57530 Human

Omim: 609473 Human

SwissProt: Q9P2M7 Human

Unigene: 591464 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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