Rabbit Anti-GTF2F1/FITC Conjugated antibody

Home > Product > Antibody > Rabbit Anti-GTF2F1/FITC Conjugated antibody

TFIIF RAP30; ATP dependent helicase GTF2F2; BTF 4; BTF4; General transcription factor IIF polypeptide 1; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF polypeptide 1 74kDa; General transcription fac

Cat:

SL8479R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human TFIIF RAP30

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

28kDa

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

Function:
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.

Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.

Subcellular Location:
Nuclear

Similarity:
Belongs to the TFIIF beta subunit family.

Database links:

Entrez Gene: 2962 Human

Entrez Gene: 505702 Cow

Entrez Gene: 2963 Human

Entrez Gene: 68705 Mouse

Entrez Gene: 19653 Mouse

Entrez Gene: 316123 Rat

Entrez Gene: 81674 Rat

Omim: 189968 Human

Omim: 189969 Human

SwissProt: Q5EA53 Cow

SwissProt: P13984 Human

SwissProt: P35269 Human