Rabbit Anti-CPSF2/FITC Conjugated antibody

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Cleavage and polyadenylation specific factor 2; Cleavage and polyadenylation specificity factor 100 kDa subunit; Cleavage and polyadenylation specificity factor subunit 2; CPSF 100 kDa subunit; CPSF 2; CPSF100; KIAA1367.

Cat:

SL14036R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CPSF2

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

88kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
CPSF contains four subunits and localizes to the nucleoplasm where it recognizes the AAUAAA signal in pre-mRNA and interacts with other proteins to facilitate RNA cleavage and poly(A) synthesis (1,4,5). The human CPSF2 gene maps to chromosome 14q32.12 and encodes the second largest subunit of cleavage and polyadenylation specificity factor. U1 snRNP-A protein (U1A) interacts with and affects the activity of CPSF by stabilizing the interaction of CPSF with the AAUAAA-containing RNAs to increase the efficiency of polyadenylation .

Function:
CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.

Subcellular Location:
Nuclear.

Database links:

Entrez Gene: 53981 Human

Entrez Gene: 51786 Mouse

Entrez Gene: 299256 Rat

Omim: 606028 Human

SwissProt: Q9P2I0 Human

SwissProt: O35218 Mouse

SwissProt: D3Z9E6 Rat

Unigene: 657632 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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