Rabbit Anti-DDX60/FITC Conjugated antibody

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DDX60; DDX60_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 60; DEAD box protein 60; Probable ATP-dependent RNA helicase DDX60.

Cat:

SL14253R-FITC

Species Reactivity：

Human,(predicted: Mouse,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human DDX60

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

Flow-Cyt=3ug/TestICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

198kDa

Product Overview：

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-DDX60 antibody (SL14253R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
DDX60 is a 1,712 amino acid protein belonging to the helicase family. DDX60 contains one helicase ATP-binding domain and one helicase SLCterminal domain. The gene encoding DDX60 maps to human chromosome 4, which represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntington gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Chromosome 4 reportedly contains the largest gene deserts (regions of the genome with no protein encoding genes) and has one of the two lowest recombination frequencies of the human chromosomes.

Similarity:
Belongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase SLCterminal domain.

Database links:

Entrez Gene: 55601 Human

Entrez Gene: 234311 Mouse

Entrez Gene: 10072801 Rat

Omim: 613974 Human

SwissProt: Q8IY21 Human

Unigene: 591710 Human

Unigene: 275233 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-DDX60 antibody (SL14253R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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