Rabbit Anti-ECSIT/FITC Conjugated antibody

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Ecsit; ECSIT homolog; ECSIT protein; ECSIT_HUMAN; Evolutionarily conserved signaling intermediate in Toll pathway; Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; Evolutionarily conserved signaling intermediate in Tol

Cat:

SL14488R-FITC

Species Reactivity：

(predicted: Human,Mouse,Rat,Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ECSIT

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

44kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
ECSIT is a 431 amino acid ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. Within the Toll pathway, ECSIT regulates MEKK1 processing for activation of NFκB, a major event leading to initiation of the innate immune response. In the mitochondria, ECSIT interacts with NDUFAF1 and assists in the formation of NADH:ubiquinone oxidoreductase (complex I), an extremely complicated multiprotein complex located in the inner mitochondrial membrane that functions in the transport of electrons from NADH to ubiquinone. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. There are two isoforms of ECSIT that are produced as a result of alternative splicing events

Function:
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.

Subunit:
Interacts with MAP3K1, SMAD4 and TRAF6. Interacts with SMAD1 only after BMP4-treatment (By similarity). Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 (By similarity). Interacts with NDUFAF1.

Subcellular Location:
Cytoplasm. Nucleus. Mitochondrion.

Similarity:
Belongs to the ECSIT family.

Database links:

Entrez Gene: 51295 Human

Entrez Gene: 26940 Mouse

Entrez Gene: 300447 Rat

Omim: 608388 Human

SwissProt: Q9BQ95 Human

SwissProt: Q9QZH6 Mouse

SwissProt: Q5XIC2 Rat

Unigene: 515146 Human

Unigene: 425568 Mouse

Unigene: 8455 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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