Rabbit Anti-MED28/FITC Conjugated antibody

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EG1; Endothelial-derived protein 1; FKSG20; Magicin; med28; MED28_HUMAN; Mediator complex subunit 28; Mediator of RNA polymerase II transcription subunit 28; Mediator of RNA polymerase II transcription subunit 28; Mediator of RNA polymerase II tr

Cat:

SL18767R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MED28

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

20kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Med28 is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm. Widely expressed with highest expression in vascular tissues such as placenta, testis and liver, Med28 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in signal regulation and cell proliferation. It is suggested that Med28 functions as a repressor of SMC (smooth muscle cell) differentiation and may be associated with abnormalities in SMC growth and differentiation, including atherosclerosis, asthma, hypertension and smooth muscle tumors.

Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

Subcellular Location:
Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

Tissue Specificity:
Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.

Similarity:
Belongs to the Mediator complex subunit 28 family.

Database links:

Entrez Gene: 80306 Human

Entrez Gene: 66999 Mouse

Omim: 610311 Human

SwissProt: Q9H204 Human

SwissProt: Q920D3 Mouse

Unigene: 434075 Human

Unigene: 644788 Human

Unigene: 283045 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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