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Rabbit Anti-Secretogranin V/FITC Conjugated antibody
background:
Sg V is a 212 amino acid protein that is secreted by endocrine and neuroendocrine secretory granules and belongs to the 7B2 family. Existing as two alternatively spliced isoforms, Sg V interacts with PC2 and, via this interaction, functions as a molecular chaperone for PC2, effectively preventing its premature activation in regulated secretory pathways. More specifically, Sg V binds to PC2 and facilitates its transport from the endoplasmic reticulum to secretory compartments, thus allowing PC2 to be cleaved and activated during the correct phase of the regulated secretory pathway. Sg V is subject to post-translational sulfation on specific tyrosine residues and is underexpressed in medulloblastomas, suggesting a role in tumor suppression. The gene encoding Sg V maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Function:
Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a SLCterminal peptide. It is the SLCterminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable.
Subunit:
Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages.
Subcellular Location:
Secreted. Note=Neuroendocrine and endocrine secretory granules.
Tissue Specificity:
Highly expressed in brain. Expressed at much lower level in other tissues.
Post-translational modifications:
Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.
Sulfated on tyrosine residues.
Similarity:
Belongs to the 7B2 family.
Database links:
Entrez Gene: 6447 Human
Entrez Gene: 20394 Mouse
Entrez Gene: 397110 Pig
Entrez Gene: 25719 Rat
Omim: 173120 Human
SwissProt: P05408 Human
SwissProt: P12961 Mouse
SwissProt: P01165 Pig
SwissProt: P27682 Rat
Unigene: 156540 Human
Unigene: 4836 Mouse
Unigene: 6173 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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