Home > Product > Antibody > Rabbit Anti-NAGPA/FITC Conjugated antibody
Mannose 6-phosphate-uncovering enzyme; N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase; NAGPA; NAGPA_HUMAN; Phosphodiester alpha-GlcNAcase.
Cat:
SL19003R-FITC
Species Reactivity:
(predicted: Human,Mouse,Rat,Dog,Pig,Horse,)
Immunogen:
KLH conjugated synthetic peptide derived from human NAGPA
Format:
Lyophilized or Liquid
Storage instructions:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Calculated MW:
51kDa
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Unit:
Price: $
Product PDFs
Datasheet:


background:
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's SLCterminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network. [provided by RefSeq, Jul 2008]

Function:
Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step.

Subunit:
Homotetramer arranged as two disulfide-linked homodimers

Subcellular Location:
Golgi apparatus > Golgi stack membrane. Cis/medial Golgi.

Tissue Specificity:
Isoform 2 may be brain-specific.

Post-translational modifications:
The precursor is cleaved and activated in the trans-Golgi network by a furin endopeptidase.

Similarity:
Contains 1 EGF-like domain.

Database links:

Entrez Gene: 515935 Cow

Entrez Gene: 98019 Dog

Entrez Gene: 100723215 Guinea pig

Entrez Gene: 100069983 Horse

Entrez Gene: 51172 Human

Entrez Gene: 27426 Mouse

Entrez Gene: 100512596 Pig

Entrez Gene: 72476 Rat

Omim: 607985 Human

SwissProt: P68827 Cow

SwissProt: Q9UK23 Human

SwissProt: Q8BJ48 Mouse

Unigene: 21334 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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