Rabbit Anti-NDFIP2/FITC Conjugated antibody

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Ndfip2; NEDD4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain binding protein 5A; NEDD4 WW domain-binding protein 5A; NFIP2_HUMAN; Putative MAPK activating protein PM04/PM05/PM06/PM07; Putative MAPK-activating prot

Cat:

SL19059R-FITC

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NDFIP2

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 ∑C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20∑C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

36kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
NDFIP2 is a 336 amino acid protein that localizes to the membrane of both the endosome and the golgi apparatus. Expressed in kidney, heart, brain, lung, liver, placenta and skeletal muscle, Ndfip2 interacts with NEDD4 and NEDD4-L and is thought to be involved in endocytosis and in the NFⅹB and MAPK signaling pathways. Additionally, Ndfip2 may function as an adaptor protein that may recruit NEDD4 ubiquitin-protein ligases to protein trafficking machinery in the golgi. Ndfip2 is activated by T cells and may be ubiquitinated by NEDD4 or NEDD4-L, an event that does not affect Ndfip2 function. The gene encoding Ndfip2 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome.

Function:
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.

Subunit:
Forms heterodimers with NDFIP1. Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4. Interacts with NEDD4L By similarity. Interacts with PTEN. When phosphorylated at Tyr-167, interacts with SRC and LYN SH2 domain. May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation. Interacts with SLC11A2/DMT1. May interact with phosphorylated EGFR.

Subcellular Location:
Endosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane.

Tissue Specificity:
Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.

Post-translational modifications:
Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover.
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN.

Database links:

Entrez Gene: 76273 Mouse

Entrez Gene: 361089 Rat

SwissProt: Q91ZP6 Mouse

SwissProt: F1M5D2 Rat

Unigene: 290669 Mouse

Unigene: 387487 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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