Rabbit Anti-NC2 alpha/FITC Conjugated antibody

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DR1 associated corepressor; DR1 associated protein 1 (negative cofactor 2 alpha); DR1 associated protein 1; DR1 associated protein1; Dr1-associated corepressor; Dr1-associated protein 1; DRAP 1; DRAP1; NC 2 alpha; NC 2alpha; NC2 alpha; NC2-alph

Cat:

SL19041R-FITC

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NC2 alpha

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

22kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]

Function:
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Ref.

Subunit:
Heterodimer with DR1. Binds BTAF1.

Subcellular Location:
Nucleus

Tissue Specificity:
Ubiquitous. Highly expressed in adult testis, heart, skeletal muscle, pancreas and brain, and in fetal brain, liver and kidney.

Post-translational modifications:
Phosphorylation reduces DNA binding, but has no effect on heterodimerization and TBP binding.

Similarity:
Belongs to the NC2 alpha/DRAP1 family.
Contains 1 histone-fold domain.

Database links:

Entrez Gene: 10589 Human

Entrez Gene: 540345 Cow

Entrez Gene: 66556 Mouse

Entrez Gene: 293674 Rat

Omim: 602289 Human

SwissProt: Q2YDP3 Cow

SwissProt: Q14919 Human

SwissProt: Q9D6N5 Mouse

SwissProt: A0JPP1 Rat

Unigene: 356742 Human

Unigene: 275446 Mouse

Unigene: 40582 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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