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Home > Product > Antibody > Rabbit Anti-SLC25A3/FITC Conjugated antibody
mitochondrial; Mitochondrial phosphate carrier protein; MPCP_HUMAN; OK/SW cl.48; PHC; Phosphate carrier protein; Phosphate carrier protein mitochondrial; Phosphate carrier protein mitochondrial precursor; Phosphate transport protein; PTP; Slc25a
Cat:
SL19814R-FITC
Species Reactivity：
(predicted: Human,Mouse,Rat,)
Immunogen：
KLH conjugated synthetic peptide derived from human SLC25A3
Format：
Lyophilized or Liquid
Storage instructions：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer：
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ICC=1:50-200IF=1:200-800not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Calculated MW：
35kDa
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-SLC25A33 Antibody, conjugated (SL19814R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.
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Unit:
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Product PDFs
Datasheet:


background:
The protein encoded by this gene catalyzes the transport of phosphate into the mitochondrial matrix, either by proton cotransport or in exchange for hydroxyl ions. The protein contains three related segments arranged in tandem which are related to those found in other characterized members of the mitochondrial carrier family. Both the N-terminal and SLCterminal regions of this protein protrude toward the cytosol. Multiple alternatively spliced transcript variants have been isolated. [provided by RefSeq, Jul 2008]

Function:
Transport of phosphate groups from the cytosol to the mitochondrial matrix. Phosphate is cotransported with H(+).

Subunit:
Interacts with PPIF; the interaction is impaired by CsA.

Subcellular Location:
Mitochondrion inner membrane.

DISEASE:
Mitochondrial phosphate carrier deficiency (MPCD)

Similarity:
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.

Database links:

Entrez Gene: 5250 Human

Omim: 600370 Human

SwissProt: Q00325 Human

Unigene: 290404 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-SLC25A33 Antibody, conjugated (SL19814R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, SLC0033) was used to stain the cell nuclei.
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