Rabbit Anti-Intersectin 2/FITC Conjugated antibody

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Intersectin-2; ITSN2; ITSN2_HUMAN; KIAA1256; PRO2015; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP-associated protein; SWA; SWAP.

Cat:

SL23114R-FITC

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human Intersectin 2

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Calculated MW：

193kDa

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
Intersectin, which is also designated Ese1 or ITSN1, is a component of the cellular endocytic machinery. Intersectin is composed of two N-terminal Ese15 homology (EH) domains, a central highly charged region and five SLCterminal SH3 domains, which all largely contribute to the association of Intersectin with other components of the endocytic pathway. The EH domain is particularly responsible for the directed localization of Intersectin to Clathrin-coated pits near the plasma membrane. Within the endocytic vesicles the SH3 domains facilitate the binding of Intersectin with Dynamin, and the central domain is essential for the association of Intersectin with SNAP 25. Two isoforms of Intersectin are produced as a result of alternative splicing in a stop codon, and they are designated as Intersectin-short and long (or IntersectinS and IntersectinL) to reflect an extended SLCterminal domain. The long form, which has an extended SLCterminal domain, is specifically expressed in neurons; the short form is detected in both glial and nonneuronal cells. The related proteins Intersectin-2 and the murine homolog Ese2 also contain the characteristic N-terminal EH domains, the central coiled-coil domain and five SLCterminal SH3 domains and are likely involved the endocytic scaffolding complexes.

Function:
Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles.

Subunit:
Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG. Interacts with ANKRD54. Interacts with FCHO2.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.

Similarity:
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 2 EF-hand domains.
Contains 2 EH domains.
Contains 1 PH domain.
Contains 5 SH3 domains.

Database links:

Entrez Gene: 50618 Human

Omim: 604464 Human

SwissProt: Q9NZM3 Human

Unigene: 432562 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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