Mouse Anti-BCHE(1E8)/FITC Conjugated antibody

Home > Product > Antibody > Mouse Anti-BCHE(1E8)/FITC Conjugated antibody

Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase. CHLE_HUMAN.

Cat:

SLM2011M-FITC

Species Reactivity：

(predicted: Human,)

Immunogen：

Full length native protein purified from human

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Calculated MW：

66kDa

prev next

Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

background:
Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008].

Function:
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Subunit:
Homotetramer; disulfide-linked. Dimer of dimers.

Subcellular Location:
Secreted.

Tissue Specificity:
Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

DISEASE:
Butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]: Metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the type-B carboxylesterase/lipase family.

Database links:

Entrez Gene: 590 Human

Omim: 177400 Human

SwissProt: P06276 Human

Unigene: 420483 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

血清丁酰胆碱酯酶是由四个亚基组成的糖蛋白，分子量28万～35万。作为一类糖蛋白，主要存在于脑、肝、血及胆碱能神经末稍的突触间隙中，主要由肝脏合成，并存在于血清中，生理作用不明，称为假性胆碱酯酶或丁酰胆碱酯酶[1]Lockridge等采用Edman降解法测定了人血清BChE完整的氨基酸序列，共含有574个氨基酸残基。BChE氨基酸序列与其他酯酶家族蛋白有很高的同源性。以电鳐AChE的三维结构为模板对人BChE的三维结构进行计算机模拟结果表明，BChE活性中心，囊袋的底部为Ser198，其两侧相距一定距离分别是酰基结合部位和胆碱结合部位。研究发现人BChE的氨基酸序列上有Zn2+离子结合结构。

Product Feedback Wall