background:
Proliferation Marker
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985).
Subcellular Location:
Nuclear.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
增殖标志物
BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。
用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
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