background:
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Function:
Produces green light with a wavelength of 562 nm.
Subcellular Location:
Peroxisome.
Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.
Database links:
SwissProt: P08659 Firefly (Photinus pyralis)
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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