Mouse Anti-His tag/FITC Conjugated antibody

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HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.

Cat:

SLM33004M-FITC

Species Reactivity：

Human,

Immunogen：

KLH conjugated HHHHHH peptide

Format：

Lyophilized or Liquid

Storage instructions：

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

Buffer：

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

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Unit:

Price: $596.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2）可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。

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