Goat Anti-Mouse IgG H&L / Biotin antibody

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Goat Anti-Mouse IgG H&L (Biotin); Immunoglobulin G;

Cat:

SL0296G-Bio

Species Reactivity：

Mouse,

Immunogen：

Native Mouse IgG

Format：

Liquid

Storage instructions：

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Concentration：

2.0 mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IHC-P=1:200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Goat

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (SLM33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:2000) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (SLM33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:500) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (SLM33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:2000) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (SLM0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:1000) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (SLM0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:1000) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (SLM33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:500) instructionsand DAB staining.

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Unit:

Price: $36.00

Product PDFs

Datasheet: Down Datasheet

Other Information
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Protein Attributes
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Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Picture

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (SLM33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:500) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (SLM33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:2000) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (SLM0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:1000) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (SLM0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:1000) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (SLM33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 SL0296G-Bio 1:500) instructionsand DAB staining.

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