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Home > Product > Antibody > Goat Anti-Rabbit IgG H&L / HRP antibody
Goat Anti-Rabbit IgG H&L (HRP); Immunoglobulin G;
Cat:
SL0295G-HRP
Species Reactivity：
Rabbit,
Immunogen：
Native rabbit IgG
Format：
Liquid
Storage instructions：
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Concentration：
2.0 mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:10000-50000ELISA=1:50000-200000IHC-P=1:200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Goat
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (SL3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:300) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:300) instructionsand DAB staining.Immobilized rabbit IgG (SL0295P, nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (Cat. No. SL0295G-HRP).
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Product PDFs
Datasheet:


Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Picture

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (SL3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-HRP 1:300) instructionsand DAB staining.
Immobilized rabbit IgG (SL0295P, nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (Cat. No. SL0295G-HRP).
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