Rabbit Anti-PAR1 antibody | Sunlong Medical

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SAR; PAR-1; PAR 1; BXR; CF2R; F2R; HTR; TR; Proteinase-activated receptor 1 precursor; Proteinase activated receptor 1; Thrombin receptor; Coagulation factor II receptor; Prader-Willi/Angelman region-1; Coagulation factor II (thrombin) receptor; PAR1_HUMA

Cat:

SL0828R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human PAR1:251-350/425<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562(Human) Cell Lysate at 30 ugPrimary: Anti-PAR1 (SL0828R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kDSample: Brain (Rat) Lysate at 30 ugLiver (Mouse) Lysate at 30 ugPrimary: Anti- PAR-1 (SL0828R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 42kDObserved band size : 44kDSample: Lane 1: U2os (Human) Cell Lysate at 30 ugLane 2: U251 (Human) Cell Lysate at 30 ugLane 3: SH-SY5Y (Human) Cell Lysate at 30 ugLane 4: K562 (Human) Cell Lysate at 30 ugLane 5: A431 (Human) Cell Lysate at 30 ugPrimary: Anti-Thrombin receptor (SL0828R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 62 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]

Function:
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Platelets and vascular endothelial cells.

Post-translational modifications:
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P25116

Gene ID:
2149

Database links:

Entrez Gene: 2149 Human

Omim: 187930 Human

SwissProt: P56488 Baboon

SwissProt: P25116 Human

Unigene: 482562 Human

PAR蛋白全称为蛋白酶体活化受体(Protease-activated receptors)，又称：凝血酶受体(Thrombin Receptor)是独立家族的G蛋白偶联受体，研究认为与炎症，实体肿瘤等密切相关。
PAR1蛋白是乳腺癌细胞侵润和迁移的必需因子，它是作为基质金属蛋白酶体1（MMP-1）的受体关键性作用，这一研究结果为研制新的抗癌药物提供新的靶点。 Picture

Sample:
Brain (Rat) Lysate at 30 ug
Liver (Mouse) Lysate at 30 ug
Primary: Anti- PAR-1 (SL0828R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 42kD
Observed band size : 44kD

Sample:
Lane 1: U2os (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-Thrombin receptor (SL0828R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall

Specific References (12) | SL0828R has been referenced in 12 publications.

[IF=4.57] Daisuke Noguchi. et al. The impact of dabigatran treatment on sinusoidal protection against hepatic ischemia‐reperfusion injury in mice. 2020 Oct 27 WB ; Mouse.

PubMed:33108682

[IF=1.54] Akaishi T et al. The Synthetic Curcumin Derivative CNB-001 Attenuates Thrombin-Stimulated Microglial Inflammation by Inhibiting the ERK and p38 MAPK Pathways. Biol Pharm Bull. 2020;43(1):138-144. WB ; Rat.

PubMed:31902919

[IF=2.454] Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus V Golderman WB ; Mouse.

PubMed:30515700

[IF=2.81] Shimon et al. Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus. (2017) PLoS.One. 12:e0188524 WB ; Mouse.

PubMed:29182653

[IF=3.294] Posthuma JJ et al. Protease-activated receptors are potential regulators in the development of arterial endofibrosis in high-performance athletes. (2018) J Vasc Surg. IHC ; Human.

PubMed:30314721

[IF=2.8] Shimon, Marina Ben, et al. "Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus." PloS one 12.11 (2017): e0188524. WB ; Mouse.

PubMed:29182653

[IF=3.01] Liu, Rui, et al. "Identification of FLOT2 as a novel target for microRNA-34a in melanoma." Journal of Cancer Research and Clinical Oncology (2014): 1-14. WB ; Human.

PubMed:25403318

[IF=1.872] Noguchi D et al. Antiapoptotic Effect by PAR-1 Antagonist Protects Mouse Liver Against Ischemia-Reperfusion Injury. J Surg Res. 2019 Oct 22. pii: S0022-964(19)30689-4. WB ; Mouse&Human.

PubMed:31653415

[IF=2.07] Chao G et al. Teprenone ameliorates diclofenac-induced small intestinal injury via inhibiting protease activated receptors 1 and 2 activityBiomarkers.2021 Feb;26(1):38-44. IHC ; Rat.

PubMed:33176506

[IF=3.161] Shavit Stein E et al. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment.Neural Plast. 2018 Jun 19;2018:7692182. WB ; Mouse.

PubMed:30018633

[IF=2.31] Fan, Hai-Xia, et al. "Expression of MMP-1/PAR-1 and patterns of invasion in oral squamous cell carcinoma as potential prognostic markers." OncoTargets and therapy 8 (2015): 1619. IHSLCP ; Human.

PubMed:26170698

[IF=3.53] Acton, David, and Gareth B. Miles. "Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine." PLOS ONE 10.8 (2015): e0134488. IHSLCP ; Mouse.

PubMed:26252389