Rabbit Anti-Pan Cytokeratin antibody

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pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1,2,4,5,6,7,8,71,72,75,78].

Cat:

SL1712R

Species Reactivity：

Human,Mouse,Rat,Cow,(predicted: Chicken,Dog,Pig,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human cytokeratins

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Bladder (Mouse) Lysate at 40 ugPrimary: Anti-Pan Cytokeratin (SL1712R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42-64 kDObserved band size: 60 kDSample: Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugLane 3: A673 (Human) Cell Lysate at 30 ugPrimary: Anti-Pan Cytokeratin (SL1712R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40-60 kDObserved band size: 47/60 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Pan Cytokeratin) polyclonal Antibody, Unconjugated (SL1712R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control（black line）:A549. Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgGProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.

Subcellular Location:
Cytoplasmic.

Tissue Specificity:
epithelial cells

SWISS:
N/A

Gene ID:
Pan Cytokeratin

P-CK广谱细胞角蛋白（AE1/AE3）主要标记角化上皮、复层鳞状上皮、复层上皮、增生的角化上皮和单层上皮，用于鳞癌，各种腺癌、移行上皮癌，小细胞癌，恶性间皮瘤、生殖细胞肿瘤，部分滑膜肉瘤、平滑肌肉瘤等表达。 Picture

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: A673 (Human) Cell Lysate at 30 ug
Primary: Anti-Pan Cytokeratin (SL1712R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40-60 kD
Observed band size: 47/60 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Pan Cytokeratin) polyclonal Antibody, Unconjugated (SL1712R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL1712R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control（black line）:A549.
Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody (SL1712R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (16) | SL1712R has been referenced in 16 publications.

[IF=3.467] Chuanli Zhang. et al. Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes. Exp Eye Res. 2022 Feb;:108983 IF ; Human.

PubMed:10.1016/j.exer.2022.108983

[IF=7.038] Verdiana Trappetti. et al. Synchrotron Microbeam Radiotherapy for the treatment of lung carcinoma: a pre-clinical study. Int J Radiat Oncol. 2021 Aug;: WB ; Rat.

PubMed:34364976

[IF=5.736] Ryuhei Okada. et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials. Ebiomedicine. 2021 May;67:103345 IF ; Mouse.

PubMed:33933782

[IF=6.126] Yasuhiro Maruoka. et al. Interleukin-15 after Near-Infrared Photoimmunotherapy (NIR-PIT) Enhances T Cell Response against Syngeneic Mouse Tumors. Cancers. 2020 Sep;12(9):2575 IF,IHC ; Mouse.

PubMed:32927646

[IF=6.126] Wakiyama H et al. Increased Immunogenicity of a Minimally Immunogenic Tumor after Cancer-Targeting Near Infrared PhotoimmunotherapyCancers (Basel).2020 Dec 12;12(12):3747. IHC ; Mouse.

PubMed:33322807

[IF=6.014] Brechbuhl HM et al. Fibroblast subtypes define a metastatic matrisome in breast cancer. JCI Insight. 2020 Feb 27;5(4). IHSLCP ; Mouse.

PubMed:6445383

[IF=2.81] Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233 IHSLCP ; Mouse.

PubMed:28196103

[IF=1.8] Li, Xin, et al. "Enrichment of Oct3/4‐positive cells from a human bronchial epithelial cell line." Apmis 121.7 (2013): 612-625. WB ; Human.

PubMed:23216104

[IF=1.23] Cheng, Pang, et al. "Colocalization of Metastasis-associated proteins 1/2 and Estrogen receptor alpha in rat epididymis." Tissue and Cell (2017). IF(ICC) ; Rat.

PubMed:10.1016/j.tice.2017.07.006

[IF=2.75] Li, Yuming, et al. "Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus." Virus Research (2014). IHSLCF ; Pig.

PubMed:24787009

[IF=18.952] Mahmoud Labibet al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng . 2020 Jul 27. FCM ; Human.

PubMed:32719513

[IF=3.317] Tomchaney, M.. et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoSLV2 infection and disease. Bmc Pulm Med. 2021 Dec;21(1):1-14 IF ; Human, Mouse.

PubMed:34425811

[IF=4.966] Hui Ren. et al. Arecoline induces epithelial‐mesenchymal transformation and promotes metastasis of oral cancer by SAA1 expression. Cancer Sci. 2021 Jun; 112(6): 2173–2184 IHC ; Human.

PubMed:33626219

[IF=4.086] Yasuhiro Maruoka. et al. Near-Infrared Photoimmunotherapy Combined with CTLA4 Checkpoint Blockade in Syngeneic Mouse Cancer Models. Vaccines-Basel. 2020 Sep;8(3):528 IHC ; Mouse.

PubMed:32937841

[IF=2.2] Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950. WB ; Bovine.

PubMed:29062612

[IF=2.47] Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015). Human.

PubMed:26723972