Rabbit Anti-TLR4 antibody | Sunlong Medical

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TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN.

Cat:

SL20594R

Species Reactivity：

Human,Mouse,Rat,Cow,(predicted: Pig,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TLR4:701-800/839

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:1000-2000ELISA=1:1000-5000IHC-P=1:500-1000IHC-F=1:500-1000IF=1:200-1000（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562(Human) Cell Lysate at 40 ugHL-60(Human) Cell Lysate at 40 ugRAW264.7(Mouse) Cell Lysate at 40 ugPrimary:Anti-TLR4 (SL20594R) at 1/2000 dilutionSample: Lane 1: Placenta (Mouse) Lysate at 40 ugLane 2: Lymph node (Rat) Lysate at 40 ugLane 3: BV2-lps (Mouse) Cell Lysate at 30 ugLane 4: HL60 (Human) Cell Lysate at 30 ugLane 5: K562 (Human) Cell Lysate at 30 ugLane 6: A549 (Human) Cell Lysate at 30 ugPrimary: Anti-TLR4 (SL20594R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110 kDObserved band size: 135 kDSample: Lane 1: Jurkat (Human) Cell Lysate at 30 ugLane 2: HUVEC (Human) Cell Lysate at 30 ugLane 3: U-2OS (Human) Cell Lysate at 30 ugLane 4: Lymph node (Mouse) Lysate at 40 ugLane 5: Raw264.7 (Mouse) Cell Lysate at 30 ugPrimary: Anti-TLR4 (SL20594R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 100-135 kDObserved band size: 120 kDThis image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- TLR4 Polyclonal Antibody (SL20594R) at 1:300 for 3 hours at 4°C, followed by Alexa Fluor 488-conjugated secondary antibody for an additional hour.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoSLV2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]

Function:
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Post-translational modifications:
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

DISEASE:
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

Similarity:
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain

SWISS:
O00206

Gene ID:
7099

Database links:

Entrez Gene: 7099 Human

Entrez Gene: 21898 Mouse

Entrez Gene: 29260 Rat

SwissProt: O00206 Human

SwissProt: Q9QUK6 Mouse

SwissProt: Q9QX05 Rat

Unigene: 174312 Human

Unigene: 38049 Mouse

Picture

Sample:
K562(Human) Cell Lysate at 40 ug
HL-60(Human) Cell Lysate at 40 ug
RAW264.7(Mouse) Cell Lysate at 40 ug
Primary:Anti-TLR4 (SL20594R) at 1/2000 dilution

Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Rat) Lysate at 40 ug
Lane 3: BV2-lps (Mouse) Cell Lysate at 30 ug
Lane 4: HL60 (Human) Cell Lysate at 30 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Lane 6: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-TLR4 (SL20594R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 135 kD

Sample:
Lane 1: Jurkat (Human) Cell Lysate at 30 ug
Lane 2: HUVEC (Human) Cell Lysate at 30 ug
Lane 3: U-2OS (Human) Cell Lysate at 30 ug
Lane 4: Lymph node (Mouse) Lysate at 40 ug
Lane 5: Raw264.7 (Mouse) Cell Lysate at 30 ug
Primary: Anti-TLR4 (SL20594R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100-135 kD
Observed band size: 120 kD

This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- TLR4 Polyclonal Antibody (SL20594R) at 1:300 for 3 hours at 4°C, followed by Alexa Fluor 488-conjugated secondary antibody for an additional hour.

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Specific References (29) | SL20594R has been referenced in 29 publications.

[IF=2.217] Qi Wang. et al. Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets. Ital J Anim Sci. 2022;21(1):612-623 WB ; Piglets.

PubMed:10.1080/1828051X.2022.2048977

[IF=3.414] Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361. WB ; Human.

PubMed:31683033

[IF=5.717] Na Chen. et al. Casein Oligochitosan-Glycation by Transglutaminase Enhances the Anti-Inflammatory Potential of Casein Hydrolysates to the Lipopolysaccharide-Stimulated IESLC6 Cells. Nutrients. 2022 Jan;14(3):686 WB ; Rat.

PubMed:35277044

[IF=6.419] Quan Rao. et al. Dendritic cell combination therapy reduces the toxicity of triptolide and ameliorates colitis in murine models. Drug Deliv. 2022;29(1):679-691 WB ; Mouse.

PubMed:10.1080/10717544.2022.2044935

[IF=5.246] Zou Xiong. et al. Toll-Like Receptors Serve as Biomarkers for Early Diagnosis and Prognosis Assessment of Kidney Renal Clear Cell Carcinoma by Influencing the Immune Microenvironment: Comprehensive Bioinformatics Analysis Combined With Experimental Validation. Front Mol Biosci. 2022 Jan;0:24 FC ; Human.

PubMed:35127830

[IF=4.411] Shi-Qing Cai. et al. The In Vitro Anti-Inflammatory Activities of Galangin and Quercetin towards the LPS-Injured Rat Intestinal Epithelial (IESLC6) Cells as Affected by Heat Treatment. Molecules. 2021 Jan;26(24):7495 WB ; Rat.

PubMed:34946578

[IF=5.81] Xu X. et al. Alhagi pseudalhagi Extract Exerts Protective Effects Against Intestinal Inflammation in Ulcerative Colitis by Affecting TLR₄-Dependent NF-κB Signaling Pathways.. Front Pharmacol. 2021 Nov;12:76922-76922 WB ; Mouse.

PubMed:3963708

[IF=5.878] Chiara Corpetti. et al. Cannabidiol inhibits SARS-Cov-2 spike (S) protein-induced cytotoxicity and inflammation through a PPARγ-dependent TLR4/NLRP3/Caspase-1 signaling suppression in Caco-2 cell line. 2021 Oct 12 WB ; Human.

PubMed:34643000

[IF=2.1] Chen W et al. Integrated analysis of a lncRNA‑mRNA network reveals a potential mechanism underlying necrotizing enterocolitis. Mol Med Rep . 2020 Jul;22(1):423-435. WB ; human.

PubMed:32319128

[IF=3.845] Peng LY et al. Protective Effect of Piceatannol Against Acute Lung Injury Through Protecting the Integrity of Air-Blood Barrier and Modulating the TLR4/NF-κB Signaling Pathway Activation. Front Pharmacol. 2020 Jan 22;10:1613. WB ; Mouse.

PubMed:6438265

[IF=2.553] Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63. IHC ; Mouse.

PubMed:31073286

[IF=3.687] Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. WB ; Rat.

PubMed:30972748

[IF=3.432] Dong W et al. Polychlorinated biphenyl quinone induces caspase 1-mediated pyroptosis through the induction of pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD signal axis. Chem Res Toxicol. 2019 Apr 23. WB&ICF ; Human.

PubMed:30977128

[IF=2.276] Zhao Yonglin. et al. Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation through a Toll-like Receptor-Related Pathway after Diffuse Axonal Injury in Rats. Biomed Res Int. 2020;2020:5946205 WB ; Rat.

PubMed:32912838

[IF=2.984] Yitong Pan. et al. N-acetyl-L-tryptophan attenuates hepatic ischemia-reperfusion injury via regulating TLR4/NLRP3 signaling pathway in rats. Peerj. 2021 Aug;9:e11909 WB,IF ; Rat.

PubMed:34434653

[IF=4.932] Alessandro Del Re. et al. Ultramicronized Palmitoylethanolamide Inhibits NLRP3 Inflammasome Expression and Pro-Inflammatory Response Activated by SARS-CoSLV2 Spike Protein in Cultured Murine Alveolar Macrophages. Metabolites. 2021 Sep;11(9):592 WB,IF ; mouse.

PubMed:3456888

[IF=4.451] Meilan Xue. et al. Neuroprotective effect of fucoidan by regulating gut-microbiota-brain axis in alcohol withdrawal mice. J Funct Foods. 2021 Nov;86:104726 WB ; mouse.

PubMed:10.1016/j.jff.2021.104726

[IF=4.162] Jiali Yang. et al. Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Des Dev Ther. 2021 Jul;15:3163-336 IHC ; Rat.

PubMed:34321866

[IF=4.235] Zhao Xueqin. et al. The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier. Front Microbiol. 2021 Jun;12:1392 IF ; Pig.

PubMed:10.3389/fmicb.2021.644887

[IF=1.951] Li Liu. et al. Inhibition of inducible nitric oxide synthase improved erectile dysfunction in rats with type 1 diabetes. 2021 Jun 16 WB ; Rat.

PubMed:34137064

[IF=3.37] Xuelian Ma. et al. Down-regulated long non-coding RNA RMST ameliorates dopaminergic neuron damage in Parkinson’s disease rats via regulation of TLR/NF-κB signaling pathway. Brain Res Bull. 2021 Apr;: WB ; Rat.

PubMed:33933526

[IF=4.147] Sijie Yuan. et al. Bacteroides vulgatus diminishes colonic microbiota dysbiosis ameliorating lumbar bone loss in ovariectomized mice. Bone. 2021 Jan;142:115710 WB ; Mouse.

PubMed:33148507

[IF=3.69] Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275 WB ; Rat.

PubMed:32810620

[IF=1.918] Dongfeng Cai. et al. The Effects of microRNA-515-5p on the Toll-Like Receptor 4 (TLR4)/JNK Signaling Pathway and WNT1-Inducible-Signaling Pathway Protein 1 (WISP-1) Expression in Rheumatoid Arthritis Fibroblast-Like Synovial (RAFLS) Cells Following Treatment with Receptor Act. Med Sci Monitor. 2020; 26: e920611-1–e920611-9 WB ;

PubMed:32361708

[IF=2.215] Karadag Remzi. et al. Effects of Different Doses of Systemic Isotretinoin on Eyes: A Histopathological and Immunohistochemical Study in Rats. Cornea. 2020 May;39(5):621-627 WB ; Human.

PubMed:31842038

[IF=2.1] Wenjuan Chenet al. Integrated analysis of a lncRNA‑mRNA network reveals a potential mechanism underlying necrotizing enterocolitis. Mol Med Rep . 2020 Jul;22(1):423-435. WB ; Human.

PubMed:32319128

[IF=1.287] Li X et al. Leptin improves intestinal flora dysfunction in mice with high-fat diet-induced obesity. J Int Med Res. 2020 Jun;48(6):300060520920062. WB ; Mouse.

PubMed:32529176

[IF=1.984] Wang X et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-κB/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med. 2020 Feb 28;2020:4572185. ELISA ; rat.

PubMed:32328132

[IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419. WB ; Rat.

PubMed:29787988