Rabbit Anti-Fas Ligand antibody

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Tumor necrosis factor ligand superfamily member 6; FASLG; APO1L; Apoptosis (APO 1) antigen ligand 1; Fas-L; Apoptosis antigen ligand 1; APTL; CD178; CD-178; CD 178; CD95L; CD95 ligand; CD95-L; CD95L protein; FAS antigen ligand; Fas L; FASL; Fas ligand (TN

Cat:

SL0216R

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human Fas Ligand:196-281/281

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Bone (Mouse) Lysate at 40 ugLane 2: Bone (Rat) Lysate at 40 ugPrimary: Anti-Fas Ligand (SL0216R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 40 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti-Fas Ligand (SL0216R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDSample: Bone (mouse) Lysate at 40 ugPrimary: Anti- Fas ligand (SL0216R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 46 kDParaformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fas Ligand) Polyclonal Antibody, Unconjugated (SL0216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiBlank control: Mouse Kidney (blue). Primary Antibody:Rabbit Anti-phospho-Fas Ligand antibody (SL0216R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0216R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20,000 events was performed.Blank control: mouse thymouses(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Function:
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

Subunit:
Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Secreted. Cytoplasmic vesicle lumen. Lysosome lumen. Note=May be released into the extracellular fluid, probably by cleavage form the cell surface. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination.

Post-translational modifications:
N-glycosylated.
The soluble form derives from the membrane form by proteolytic processing.
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Monoubiquitinated.

DISEASE:
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
P9623

Gene ID:
356

Database links:

Entrez Gene: 356 Human

Entrez Gene: 14103 Mouse

Entrez Gene: 25385 Rat

Omim: 134638 Human

SwissProt: P9623 Human

SwissProt: P41047 Mouse

SwissProt: P36940 Rat

Unigene: 2007 Human

Unigene: 3355 Mouse

Unigene: 9725 Rat

Picture

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Fas Ligand (SL0216R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Sample: Bone (mouse) Lysate at 40 ug
Primary: Anti- Fas ligand (SL0216R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 46 kD

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fas Ligand) Polyclonal Antibody, Unconjugated (SL0216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FasL Polyclonal Antibody, Unconjugated(SL0216R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Blank control: Mouse Kidney (blue).
Primary Antibody:Rabbit Anti-phospho-Fas Ligand antibody (SL0216R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0216R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20,000 events was performed.

Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

Product Feedback Wall

Specific References (15) | SL0216R has been referenced in 15 publications.

[IF=1.918] Jisheng Wang. et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway. Med Sci Monitor. 2021; 27: e928232-1–e928232-12 WB ; Mouse.

PubMed:33589581

[IF=1.487] Yuefeng Gaoet al. Vitamin E promotes ovine Sertoli cell proliferation by regulation of genes associated with cell division and the cell cycle. Anim Biotechnol . 2020 Jul 2;1-9. IF ; sheep.

PubMed:32615852

[IF=2.868] Jimbo H et al. Fas‐FasL interaction in cytotoxic T cell‐mediated vitiligo: The role of lesional expression of tumor necrosis factor‐α and interferon‐γ in Fas‐mediated melanocyte … Exp Dermatol. 2019 Nov 1. IHSLCP ; Human.

PubMed:31675451

[IF=2.136] Liu S et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019 Jun;131:89-95. WB ; porcine.

PubMed:30965208

[IF=1.664] Wang Z et al. β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells.Oncol Lett. 2018 Oct;16(4):4519-4525. WB ; Human.

PubMed:30197674

[IF=2.766] Zhang et al. Cytochrome P450 3A1 mediates 2,2',4,4'-tetrabromodiphenyl ether-induced reduction of spermatogenesis in adult rats. (2013) PLoS.One. 8:e66301 WB ; Rat.

PubMed:23762486

[IF=5.23] Lv, Yanhong, et al. "Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma." Scientific Reports 7 (2017): 40967. WB ; Mouse.

PubMed:28102324

[IF=2.13] Shi, Yuqin, et al. "p, p′-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway." Journal of Biomedicine and Biotechnology (2009). Rat.

PubMed:19644561

[IF=1.48] Wang, Shuhua, Qingqing Tian, and Fang An. "Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells." Oncology Letters (2016). WB ; Human.

PubMed:27602105

[IF=2.67] Wang, Yi, et al. "Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar Sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187." Molecular Reproduction and Development (2015). WB ;

PubMed:26256020

[IF=3.53] Song, Xiufang, et al. "Polychlorinated biphenyl quinone metabolite promotes p53-dependent DNA damage checkpoint activation, S-phase cycle arrest and extrinsic apoptosis in human liver hepatocellular carcinoma HepG2 Cells."Chemical Research in Toxicology (2015). WB ; Human.

PubMed:26451628

[IF=5.699] Janos L. Tanyi. et al. Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer. Npj Vaccines. 2021 Mar;6(1):1-14 IHC ; Mouse.

PubMed:33723260

[IF=1.77] Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek??s disease virus infection in the bursa of Fabricius."Research in veterinary science (2012).q WB ; Chicken.

PubMed:23164636

[IF=2.87] Shi, Yuqin, et al. "β‐benzene hexachloride induces apoptosis of rat sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL." Environmental toxicology 26.2 (2011): 124-135. WB ; Rat.

PubMed:19760616

[IF=2.85] Qi, Suqin, et al. "BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways." Reproductive Toxicology 50 (2014): 108-116. WB ; Rat.

PubMed:25461909