Home > Product > Antibody > Rabbit Anti-GRP78 antibody
BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobuli
Cat:
SL1219R
Species Reactivity:
Human,Mouse,Rat,
Immunogen:
KLH conjugated synthetic peptide derived from human GRP78:251-360/654
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Intestinal(Mouse) Lysate at 30ug; Primary: Anti-GRP78 (SL1219R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size : 78 kDObserved band size :78 kDSample: Lane 1: Spleen (Rat) Lysate at 40 ugLane 2: Cerebrum (Rat) Lysate at 40 ugLane 3: U-87MG (Human) Cell Lysate at 30 ugLane 4: Hela (Human) Cell Lysate at 30 ugLane 5: HepG2 (Human) Cell Lysate at 30 ugLane 6: MDA-MB-231 (Human) Cell Lysate at 30 ugLane 7: A431 (Human) Cell Lysate at 30 ugPrimary: Anti-GRP78 (SL1219R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 74 kDSample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti- GRP78 (SL1219R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 74 kDSample: Hyperpituitarism (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugKidney (Mouse) Lysate at 40 ugPrimary: Anti- GRP78 (SL1219R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 74 kDSample: Liver(Rat) Lysate at 40 ugPrimary: Anti- GRP78 (SL1219R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 75 kDSample: U87mg(Human) Cell Lysate at 30 ugPrimary: Anti- GRP78 (SL1219R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 75 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (SL1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(SL1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Function:
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the heat shock protein 70 family.

SWISS:
P11021

Gene ID:
3309

Database links:

Entrez Gene: 3309 Human

Entrez Gene: 14828 Mouse

Entrez Gene: 25617 Rat

Omim: 138120 Human

SwissProt: P11021 Human

SwissProt: P20029 Mouse

SwissProt: P06761 Rat

Unigene: 605502 Human

Unigene: 743241 Human

Unigene: 330160 Mouse

Unigene: 47036 Mouse

Unigene: 11088 Rat



葡萄糖调节蛋白78是热休克蛋白70家族的成员之一,又称:热休克蛋白70蛋白5,作为一种分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.它在多种肿瘤组织中呈高表达. Picture

Sample: Intestinal(Mouse) Lysate at 30ug;
Primary: Anti-GRP78 (SL1219R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 78 kD
Observed band size :78 kD
Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: U-87MG (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-GRP78 (SL1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (SL1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Hyperpituitarism (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (SL1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti- GRP78 (SL1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Sample:
U87mg(Human) Cell Lysate at 30 ug
Primary: Anti- GRP78 (SL1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (SL1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(SL1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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