Rabbit Anti-laminin antibody

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LAMA 1; LAMA; LAMA1; LAMB1; Laminin A chain; Laminin alpha 1; Laminin alpha 1 chain; Laminin B1 chain; Laminin subunit beta 1.

Cat:

SL0821R

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human laminin:901-1000/3075

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (SL0821R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (SL0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (SL0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in tandem arrays; the domain contains 4 disulfide bonds (loops a-d) the first three resemble epidermal growth factor (EGF); the number of copies of this domain in the different forms of laminins is highly variable ranging from 3 up to 22 copies.

Function:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Subcellular Location:
Secreted > extracellular space > extracellular matrix > basement membrane.

Tissue Specificity:
Broadly expressed in: skin, heart, lung, and the reproductive tracts.

Similarity:
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.

SWISS:
P25391

Gene ID:
284217

Database links:

Entrez Gene: 10319 Human

Entrez Gene: 284217 Human

Entrez Gene: 3908 Human

Entrez Gene: 3912 Human

Entrez Gene: 3913 Human

Entrez Gene: 3915 Human

Entrez Gene: 3918 Human

Entrez Gene: 16772 Mouse

Entrez Gene: 316758 Rat

Omim: 15064 Human

SwissProt: P07942 Human

SwissProt: P4843 Human

SwissProt: P25391 Human

SwissProt: P55268 Human

SwissProt: Q9Y6N6 Human

SwissProt: P19137 Mouse

SwissProt: Q9R0B6 Mouse

Unigene: 20365 Human

Unigene: 270364 Human

Unigene: 302362 Mouse

层粘连蛋白主要存在于基膜(basal lamina)结构中，是基膜所特有的非胶原糖蛋白。
层粘连蛋白在细胞发育过程中刺激细胞粘着、细胞运动。LN能够刺激胚胎中神经轴的生长,并促进成年动物的神经损伤后重生长和再生。如同纤粘连蛋白,细胞外的LN能够影响细胞的生长、迁移和分化。LN在原生殖细胞的迁移中起关键作用。
层粘连蛋白是细胞外基底膜的主要组成部分，分布于基底膜和细胞外基质内、成纤维细胞、内皮细胞和平滑肌细胞分泌层粘连蛋白。肿瘤的浸润和转移与其遭到破坏相关。该抗体可用于标记基底膜，有助于观察肿瘤周围基底膜的分布及其改变，间接提示该肿瘤的分化程度和浸润情况。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (SL0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (SL0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(SL0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall

Specific References (8) | SL0821R has been referenced in 8 publications.

[IF=4.2] Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017). IHSLCP ; Rat.

PubMed:28851916

[IF=5.959] Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852. IHF ; Mouse.

PubMed:31699966

[IF=4.19] Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015). WB ; Human.

PubMed:25742418

[IF=5.116] Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16 IF,IHC ; Rat.

PubMed:33712079

[IF=4.831] Zhang Q et al. CTRP13 attenuates the expression of LN and CASLV1 Induced by high glucose via CaMKKβ/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499. WB ; Rat.

PubMed:32554851

[IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062. IHSLCP ; Rat.

PubMed:doi:10.1016/j.jff.2020.104062

[IF=0] Ijima H et al. Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver. Gels, 2018 4(2), 39. IHF ; Pig.

PubMed:10.3390/gels4020039

[IF=8.32] Gong, Tao, et al. "A Dynamically Tunable, Bioinspired Micropatterned Surface Regulates Vascular Endothelial and Smooth Muscle Cells Growth at Vascularization." Small (2016). IHSLCP ; Rabbit.

PubMed:27595865