Rabbit Anti-Tyrosine Hydroxylase antibody

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Tyrosine Hydroxylase; DYT14; DYT5b; ple; Protein Pale; c; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyk2; TY3H_HUMAN; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH.

Cat:

SL0016R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human TH:101-165/528

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: adipocyte (mouse) Lysate at 5-10 ugmodel A, model B, model C are from different mice; Primary: Anti-Tyrosine Hydroxylase(SL0016R) at 1/2000 dilutionPredicted band size: 60 kDObserved band size: 60 kDSample: PSLC3 (Mouse) Lysate at 30 ugPrimary: Anti-Tyrosine Hydroxylase (SL0016R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1:5000 dilution; Predicted band size:60 kD Observed band size:60 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated(SL0016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Function:
Plays an important role in the physiology of adrenergic neurons.

Tissue Specificity:
Mainly expressed in the brain and adrenal glands.

DISEASE:
Defects in TH are the cause of Segawa syndrome autosomal recessive (ARSEGS) [MIM:605407]. A form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.

Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

SWISS:
P07101

Gene ID:
7054

Database links:

Entrez Gene: 7054 Human

Entrez Gene: 21823 Mouse

Entrez Gene: 25085 Rat

Omim: 191290 Human

SwissProt: P07101 Human

SwissProt: P24529 Mouse

SwissProt: P04177 Rat

Unigene: 435609 Human

Unigene: 1292 Mouse

Unigene: 11082 Rat

神经细胞标志物
酪氨酸羟化酶（TH）是儿茶酚胺类神经递质即多巴胺、去甲肾上腺素、肾上腺素生物合成过程所需的限速酶，它以四氢生物喋呤啶(SH4)为辅酶，催化酪氨酸的羟化而生成多巴(DOPA)。
已知在患帕金森病(Parkinson disease,PD)时，脑内多巴胺(dopamine,DA)的减少与此酶活性低下有关。因此对PD模型动物来说，若将TH基因植入脑内，便可以提高脑内DA水平而达到基因治疗目的。 Picture

Sample: PSLC3 (Mouse) Lysate at 30 ug
Primary: Anti-Tyrosine Hydroxylase (SL0016R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1:5000 dilution;
Predicted band size:60 kD
Observed band size:60 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated(SL0016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

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