Rabbit Anti-XIAP/BIRC4 antibody

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X-linked Inhibitor of Apoptosis Protein; RIAP-3; Baculoviral IAP repeat-containing protein 4; E3 ubiquitin-protein ligase XIAP; Inhibitor of apoptosis protein 3; X-linked IAP; IAP-like protein; HILP; BIRC4; HILP; XLP2; ILP1; Xiap; MIHA; hILP; IAP3; API3;

Cat:

SL1281R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human XIAP:201-330/496

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Skin(Mouse) Lysate at 40 ugPrimary: Anti-XIAP/BIRC4(SL1281R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 53 kDSample:Lymph node(Mouse) Lysate at 40 ugBone(Mouse) Lysate at 40 ugPrimary: Anti-XIAP/BIRC4(SL1281R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 53 kDSample:Liver(Mouse) Lysate at 40 ugPrimary: Anti-XIAP/BIRC4(SL1281R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 53 kDSample: Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti-XIAP/BIRC4 (SL1281R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDTissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-XIAP/BIRC4 Polyclonal Antibody, Unconjugated(SL1281R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiTissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Antibody incubation with (XIAP/BIRC4) polyclonal Antibody, Unconjugated (SL1281R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (Black line): HUVEC(Black). Primary Antibody (green line): Rabbit Anti-XIAP antibody (SL1281R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

Function:
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.

Subunit:
Monomer, and homodimer. Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of MAP3K7IP1 and AIFM1. Interacts with TCF25.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous, except peripheral blood leukocytes.

Post-translational modifications:
Ubiquitinated and degraded by the proteasome in apoptotic cells. Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.

DISEASE:
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

Similarity:
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.

SWISS:
Q60989

Gene ID:
331

Database links:

Entrez Gene: 331 Human

Entrez Gene: 11798 Mouse

Entrez Gene: 63879 Rat

Omim: 300079 Human

SwissProt: P98170 Human

SwissProt: Q60989 Mouse

SwissProt: Q9R0I6 Rat

Unigene: 356076 Human

Unigene: 259879 Mouse

Unigene: 91239 Rat

Picture

Sample:
Lymph node(Mouse) Lysate at 40 ug
Bone(Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4(SL1281R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD

Sample:Liver(Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4(SL1281R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD

Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4 (SL1281R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-XIAP/BIRC4 Polyclonal Antibody, Unconjugated(SL1281R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Antibody incubation with (XIAP/BIRC4) polyclonal Antibody, Unconjugated (SL1281R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control (Black line): HUVEC(Black).
Primary Antibody (green line): Rabbit Anti-XIAP antibody (SL1281R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (8) | SL1281R has been referenced in 8 publications.

[IF=3.743] Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096. Immunostaining ; human.

PubMed:32179199

[IF=1.851] Chen J et al. SM‑164 enhances the antitumor activity of adriamycin in human U2‑OS cells via downregulation of X‑linked inhibitor of apoptosis protein. Mol Med Rep. 2019 Jun;19(6):5079-5086. WB ; Human.

PubMed:31059038

[IF=1.48] Chen, Xin Mei, et al. "Ampelopsin induces apoptosis by regulating multiple c‑Myc S‑phase kinase‑associated protein 2 F‑box and WD repeat‑containing protein 7 histone deacetylase 2 pathways in human lung adenocarcinoma cells." Molecular Medicine Reports. WB ; Human.

PubMed:25333250

[IF=5.778] Lei Tian. et al. Acute ozone exposure can cause cardiotoxicity: Mitochondria play an important role in mediating myocardial apoptosis. Chemosphere. 2021 Apr;268:128838 WB ; Rat.

PubMed:33162165

[IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608. IHSLCP ; Pig.

PubMed:22023076

[IF=1.55] Jin, Gongsheng, et al. "Smac mimetic‑induced caspase‑independent necroptosis requires RIP1 in breast cancer." Molecular Medicine Reports (2015). other ;

PubMed:26573429

[IF=2.71] Sun, Yiming, et al. "Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer." Journal of Bioenergetics and Biomembranes (2015): 1-11. WB ; Human.

PubMed:2605876

[IF=3.73] Chen, Xin-mei, et al. "Wogonin Has Multiple Anti-Cancer Effects by Regulating c-Myc/SKP2/Fbw7α and HDAC1/HDAC2 Pathways and Inducing Apoptosis in Human Lung Adenocarcinoma Cell Line A549." PLOS ONE 8.11 (2013) WB ; Human.

PubMed:24265759