Rabbit Anti-PARP1 antibody | Sunlong Medical

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ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family

Cat:

SL2138R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human PARP:201-300/1014

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=0.2μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Raji Cell (Human) Lysate at 30 ugPrimary: Anti- PARP (SL2138R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 111 kDObserved band size: 111 kDSample: Lane 1: 293T (Human) Cell Lysate at 30 ugLane 2: HL60 (Human) Cell Lysate at 30 ugLane 3: K562 (Human) Cell Lysate at 30 ugLane 4: SH-SY5Y (Human) Cell Lysate at 30 ugLane 5: U251 (Human) Cell Lysate at 30 ugLane 6: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-PARP (SL2138R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kDSample: Hela(Human) Cell Lysate at 30 ugRaji(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugK562(Human) Cell Lysate at 30 ugPrimary: Anti- PARP (SL2138R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 111 kDObserved band size: 111 kDSample: Hela Cell (Human) Lysate at 30 ugPrimary: Anti- PARP (SL2138R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 111 kDObserved band size: 111 kDProtein:HepG2 lysate at 30ug; Primary: Anti-PARP (SL2138R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size:111 kDObserved band size:111 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse intestines); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): HL60 cells (blue). Primary Antibody (green line): Rabbit Anti- PARP antibody (SL2138R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control:293T. Primary Antibody (green line): Rabbit Anti-PARP1 antibody (SL2138R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit:
Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location:
Mitochondrion outer membrane; Single-pass membrane protein.
Nucleus membrane; Single-pass membrane protein.
Endoplasmic reticulum membrane; Single-pass membrane protein.
Nucleus.

Post-translational modifications:
Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity:
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.

SWISS:
P09874

Gene ID:
142

Database links:

Entrez Gene: 142 Human

Entrez Gene: 11545 Mouse

Entrez Gene: 25591 Rat

Omim: 173870 Human

SwissProt: P09874 Human

SwissProt: P11103 Mouse

SwissProt: P27008 Rat

Unigene: 177766 Human

Unigene: 277779 Mouse

Unigene: 11327 Rat

PARP(poly ADP-ribose polymerase)是DNA修复酶。
PARP是细胞凋亡核心成员半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA损伤修复与细胞凋亡中发挥着重要作用。Anti-PARP p85 是特意的PARPp85片段的特异抗体，由caspase剪切116kDa完整分子而得到的。
PARP是存在于多数真核细胞中的一个多功能蛋白质翻译后修饰酶。它通过识别结构损伤的DNA片段而被激活，对聚腺苷二磷酸核糖聚合酶PARP被认为是DNA损伤的感受器。它还能对许多核蛋白进行聚腺苷二磷酸核糖基化。因此，在DNA损伤修复与细胞凋亡中发挥着重要作用，端锚聚合酶在癌细胞端粒结构的调控机制中有重要作用。 Picture

Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug
Lane 2: HL60 (Human) Cell Lysate at 30 ug
Lane 3: K562 (Human) Cell Lysate at 30 ug
Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-PARP (SL2138R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- PARP (SL2138R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD

Sample:
Hela Cell (Human) Lysate at 30 ug
Primary: Anti- PARP (SL2138R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD

Protein:HepG2 lysate at 30ug;
Primary: Anti-PARP (SL2138R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size:111 kD
Observed band size:111 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse intestines); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (SL2138R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control (blue line): HL60 cells (blue).
Primary Antibody (green line): Rabbit Anti- PARP antibody (SL2138R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:293T.
Primary Antibody (green line): Rabbit Anti-PARP1 antibody (SL2138R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (17) | SL2138R has been referenced in 17 publications.

[IF=2.72] Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22 WB ; Human.

PubMed:34811765

[IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12 WB ; Mouse.

PubMed:33431863

[IF=2.842] Chen J et al. Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats. Diabetes Metab Syndr Obes . 2020 May 20;13:1725-1731. IHSLCP ; Rat.

PubMed:32547134

[IF=4.925] Yuan P et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology . 2020 Oct;14(8):1137-1155. WB ; Human.

PubMed:32916084

[IF=4.522] Han S et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2019 Oct 21. WB ; Chicken.

PubMed:31637727

[IF=3.098] Fan Y et al. S5, a Withanolide Isolated from Physalis Pubescens L., Induces G2/M Cell Cycle Arrest via the EGFR/P38 Pathway in Human Melanoma A375 Cells.(2018) Molecules 23(12) WB ; Human.

PubMed:30513793

[IF=1.45] Omar, S. S., R. G. Aly, and N. M. Badae. "Vitamin E improves testicular damage in streptozocin‐induced diabetic rats, via increasing vascular endothelial growth factor and poly (ADP‐ribose) polymerase‐1." Andrologia (2017). IHSLCP ; Rat.

PubMed:29164711

[IF=12.88] Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS nano (2015). WB ; Mouse.

PubMed:26389709

[IF=4.432] Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585 WB ; Human.

PubMed:3467848

[IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646 WB ; rat.

PubMed:34536814

[IF=3.367] Lijin Guo. et al. Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis. Front Physiol. 2020; 11: 573676 WB ; Chicken.

PubMed:33192575

[IF=3.15] Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189. WB ; Human.

PubMed:33276757

[IF=1.445] Li YH et al. Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/β-Catenin Signaling. Chin J Integr Med. 2020 Jan 4. WB ; Mouse&Rat.

PubMed:31903532

[IF=1.984] Wei J et al. Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Evid Based Complement Alternat Med. 2019 Oct 27;2019:272367. WB ; Rat.

PubMed:31781263

[IF=3.571] Nandi S et al. Characterization and inception of a triterpenoid astrakurkurol, as a cytotoxic molecule on human hepatocellular carcinoma cells, Hep3B. J Agric Food Chem. 2019 Jul 10;67(27):7660-7673. WB ; Human.

PubMed:31250646

[IF=0] Zhou et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. (2012) Cancers.(Basel). 4:1318-32 WB ; Human.

PubMed:24213508

[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat.

PubMed:27665780