Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody

Home > Product > Antibody > Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody

CIntegrin alpha 5 beta 3; D51 + CD61; CD51; CD61; GP3A; GPIIIa; integrin alpha v; integrin beta 3; intregrin alpha v beta 3; ITGAV; ITGB3; Platelet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; VNRA; Antigen identified by monoclonal anti

Cat:

SL1310R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 3

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HUVEC (human)Cell Lysate at 40 ugPrimary: Anti- Integrin Alpha V + Beta 3 (CD51+CD61) (SL1310R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 116 kDObserved band size: 120 kDTissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingcell: mouse lung. Incubation: Avoid light, at 4°C for 40 minutes. Red line: Blank control (mouse lung cells),2X10^6/ml, at 4°C for 40 minutes. Green line: (primary antibody) Integrin Alpha V + Beta 3 (CD51+CD61) (SL1310R), (secondary antibody)Goat Anti-rabbit IgG/FITC (SL0295G-FITC), 1:00, at 4°C for 40 minutes. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3(CD51+CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: U937(blue). Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody(SL1310R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL1310R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Cell: MCF-7Concentration:1:100Host/Isotype:Rabbit/IgGFlow cytometric analysis of Rabbit IgG isotype control (Cat#: SL1310R) on MCF-7(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.

Subcellular Location:
Cell Membrane; single-pass type I membrane protein.

SWISS:
P06756

Gene ID:
3685

Database links:

Entrez Gene: 3685 Human

SwissProt: P06756 Human

Picture

Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

cell: mouse lung.
Incubation: Avoid light, at 4°C for 40 minutes. Red line: Blank control (mouse lung cells),2X10^6/ml, at 4°C for 40 minutes. Green line: (primary antibody) Integrin Alpha V + Beta 3 (CD51+CD61) (SL1310R), (secondary antibody)Goat Anti-rabbit IgG/FITC (SL0295G-FITC), 1:00, at 4°C for 40 minutes.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin Alpha V + Beta 3(CD51+CD61) Polyclonal Antibody, Unconjugated(SL1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: U937(blue).
Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody(SL1310R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL1310R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

Cell: MCF-7
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL1310R) on MCF-7(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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Specific References (31) | SL1310R has been referenced in 31 publications.

[IF=6.4] Rui-Qin Yang. et al. Development and Preclinical Evaluation of a Near-Infrared Fluorescence Probe Based on Tailored Hepatitis B Core Particles for Imaging-Guided Surgery in Breast Cancer. Int J Nanomed. 2022; 17: 1343–172 FC ; Human.

PubMed:35345784

[IF=2.47] Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015). WB ; Human.

PubMed:26723972

[IF=3.06] Zhang, Li-xue. et al. Expression of SGLT1 in the Mouse Endometrial Epithelium and its Role in Early Embryonic Development and Implantation. 2021 Aug 30 WB ; Mouse.

PubMed:3492091

[IF=15.881] Kuo Zhang. et al. A Monotargeting Peptidic Network Antibody Inhibits More Receptors for Anti-Angiogenesis. Acs Nano. 2021;XXXX(XXX):XXX-XXX IF ; Rabbit.

PubMed:10.1021/acsnano.1c02194

[IF=3.073] Ozlem Ozen Karakus. et al. Discovery of dual targeting PEGylated BG-P1600-TAT to norepinephrine transporter (NET) and thyrointegrin αvβ3 in the treatment of neuroblastoma. Bioorgan Med Chem. 2021 Aug;43:116278 Mouse.

PubMed:34157571

[IF=4.556] Jaya Aseervathamet al. Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci . 2020 Jul 2;21(13):4734. WB ; Human.

PubMed:32630820

[IF=4.556] Jiang L et al. Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Mol Pharm. 2019 Mar 4;16(3):1350-1357. IF ; Mouse.

PubMed:30742442

[IF=4.26] Ma et al. Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis. (2017) Sci.Rep. 7:4978 other ; Human.

PubMed:28694452

[IF=2.81] Li et al. Effects of Cationic Microbubble Carrying CD/TK Double Suicide Gene and αVβ3 Integrin Antibody in Human Hepatocellular Carcinoma HepG2 Cells. (2016) PLoS.On. 11:e0158592 FCM ; Human.

PubMed:27391603

[IF=3.8] Chen, Li, et al. "Pathways of production and delivery of hepatocyte exosomes." Journal of Cell Communication and Signaling (2017): 1-15. IF(ICC) ; Mouse.

PubMed:29063370

[IF=2.829] Li, Yin, Lin Xiong, and Jianping Gong. "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." Am J Transl Res 9.6 (2017): 2865-2877. IHSLCF ; Mouse.

PubMed:28670375

[IF=3.55] Bian, Qin, et al. "Mechanosignaling activation of TGFβ maintains intervertebral disc homeostasis." Bone Research 5 (2017): 17008 IHSLCP ; Mouse.

PubMed:28392965

[IF=3.375] Park CR et al. Conjugation of arginylglycylaspartic acid to Human serum albumin decreases the tumor-targeting effect of albumin by hindering its secreted protein acidic and rich in cysteine-mediated accumulation in tumors. Am J Transl Res. 2020 Jun 15;12(6):2488-2498. IHF ; Human.

PubMed:32655786

[IF=12.121] Gehua Zhen. et al. Mechanical stress determines the configuration of TGFβ activation in articular cartilage. Nat Commun. 2021 Mar;12(1):1-16 IHC ; Mouse.

PubMed:33731712

[IF=4.566] Yun Long. et al. GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation. Front Physiol. 2021; 12: 674924 WB ; Mouse.

PubMed:34248664

[IF=6.081] Ting Li. et al. Albumin Fusion at the N-Terminus or SLCTerminus of HM-3 Leads to Improved Pharmacokinetics and Bioactivities. Biomedicines. 2021 Sep;9(9):1084 Other ; Mouse.

PubMed:10.3390/biomedicines9091084

[IF=6.603] Dan Lin. et al. iRGD-modified exosomes effectively deliver CPT1A siRNA to colon cancer cells, reversing oxaliplatin resistance by regulating fatty acid oxidation. 2021 Jul 02 IHC ; Human.

PubMed:34213835

[IF=3.706] Kosuke Mitsuyuki. et al. Evaluation of Integrin αvβ3 Expression in Murine Xenograft Models: [68Ga]Ga-DOTA-C(RGDfK) PET Study with Immunohistochemical Confirmation. Diagnostics. 2021 Jul;11(7):1295 IHC ; Mouse.

PubMed:34359378

[IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;: WB ; Mouse.

PubMed:33711525

[IF=5.442] Yusuke Mochizuki. et al. Telomerase-specific oncolytic immunotherapy for promoting efficacy of PD-1 blockade in osteosarcoma. 2020 Nov 05 FC ; Mouse.

PubMed:33151368

[IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039 WB,IF,IHC ; Mouse.

PubMed:33511306

[IF=6.205] Ozlem Ozen Karakus et al. Dual Targeting of Norepinephrine Transporter (NET) Function and Thyrointegrin αvβ3 Receptors in the Treatment of Neuroblastoma. J Med Chem. 2020 Jul 23;63(14):7653-7662. Other ;

PubMed:32571015

[IF=6.638] Wong L et al. Substrate Stiffness Directs Diverging Vascular Fates. Acta Biomater. 2019 Jul 19. pii: S1742-7061(19)30519-7. Inhibitor assay ; Mouse.

PubMed:31326665

[IF=2.561] Chaudhary P et al. HGF promotes HTR-8/SVneo cell migration through activation of MAPK/PKA signaling leading to up-regulation of WNT ligands and integrins that target β-catenin. Mol Cell Biochem. 2019 Mar;453(1-2):11-32. ICF ; Human.

PubMed:30136190

[IF=0] Tekabe Y et al. Imaging VEGF Receptors and α v β 3 Integrins in a Mouse Hindlimb Ischemia Model of Peripheral Arterial Disease.World Molecular Imaging Society, 2018 IF ; Mouse.

PubMed:10.1007/s11307-018-1191-1

[IF=6.82] Shimojo et al. Tenascin-C may accelerate cardiac fibrosis by activating macrophages via the integrin αVβ3/nuclear factor-κB/interleukin-6 axis. (2015) Hypertension. 66:757-66 other ; Mouse.

PubMed:26238448

[IF=3.31] Zhang, Yingying, et al. "Synthesis and Bioevaluation of Iodine-131 Directly Labeled Cyclic RGD-PEGylated Gold Nanorods for Tumor-Targeted Imaging." Contrast Media & Molecular Imaging 2017 (2017). IF(ICC) ; Mouse.

PubMed:29434531

[IF=3.52] Chen, Li, and David R. Brigstock. "Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC‐derived exosomes." FEBS letters (2016). other ; Mouse.

PubMed:27714787

[IF=5.56] Jiang, Lei, et al. "64Cu-Labeled Divalent Cystine Knot Peptide for Imaging Carotid Atherosclerotic Plaques." Journal of Nuclear Medicine (2015): jnumed-115. IHSLCF ; Mouse.

PubMed:25908832

[IF=7.6] Mondal, Goutam, Sugata Barui, and Arabinda Chaudhuri. "The relationship between the cyclic-RGDfK ligand and αvβ3 integrin receptor." Biomaterials (2013). Human.

PubMed:23702147