Rabbit Anti-Gamma-Adaptin antibody

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Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP 1 gamma 1 subunit; Adaptor protein complex AP 1 subunit gamma 1; Adaptor protein complex AP-1 subunit gamma-1; Adaptor related protein complex 1 gamma 1 subunit; adaptor-related

Cat:

SL1600R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Gamma-Adaptin:721-822/822

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugJurkat(Human) Cell Lysate at 30 ugPrimary: Anti-Gamma-Adaptin (SL1600R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 91 kDObserved band size: 91 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti- Gamma-Adaptin, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingCell culture(MCF7); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes and DAPI staining.Cell culture(SW96); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes and DAPI staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.

Function:
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit:
Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain).

Subcellular Location:
Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.

SWISS:
P22892

Gene ID:
164

Database links:

Entrez Gene: 164 Human

Entrez Gene: 11765 Mouse

Entrez Gene: 171494 Rat

Omim: 603533 Human

SwissProt: O43747 Human

SwissProt: P22892 Mouse

Unigene: 461253 Human

Unigene: 37210 Mouse

Unigene: 430782 Mouse

Unigene: 471663 Mouse

Unigene: 9446 Rat

γ-Adaptin参与披网格蛋白小泡组装的一种蛋白质, 分子量为100kDa, 在披网格蛋白小泡组装中与受体的细胞质结构域相互作用, 起衔接作用。有两种类型衔接蛋白, AP1参与反面高尔基体的披网格蛋白小泡的组装, AP2则参与从细胞质膜形成的披网格蛋白的组装。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti- Gamma-Adaptin, Unconjugated(SL1600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Cell culture(MCF7); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes and DAPI staining.

Cell culture(SW96); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (SL1600R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes and DAPI staining.

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