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Home > Product > Antibody > Rabbit Anti-GLUR2 antibody
Ionotropic Glutamate receptor 2; Gria2; Glur-2; GluR-B; Glur2; GluR-2; GluR-K2; Glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA receptor subunit ; AMPA-selective glutamate receptor
Cat:
SL1798R
Species Reactivity：
Human,Mouse,Rat,(predicted: Dog,)
Immunogen：
KLH conjugated synthetic peptide derived from human GluR2 :151-250/883<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: HepG2 Cell Lysate at 30 ugPrimary: Anti-GluR2 (SL1798R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 97 kDObserved band size: 117 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (SL1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (SL1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated(SL1798R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: RSC96(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ugl in 100 uL1X PBS containing 0.5% BSA(green).Blank control: RSC96(blue)Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).Blank control: RSC96(blue)Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
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Product PDFs
Datasheet:


Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing(CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.

Function:
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Subunit:
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with ATAD1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts binding to ATAD1 and to GRIP1 and leads to AMPAR complex disassembly. Interacts with PICK1 (via PDZ domain). Interacts with PRKCABP and GRIP2. Interacts with GRIA1 and SYNDIG1. Interacts with LRFN1. Found in a complex with GRIA1, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.

SWISS:
P42262

Gene ID:
2891

Database links:

Entrez Gene: 2891 Human

Entrez Gene: 2960 Mouse

Entrez Gene: 29627 Rat

Omim: 138247 Human

SwissProt: P42262 Human

SwissProt: P23819 Mouse

SwissProt: P19491 Rat

Unigene: 32763 Human

Unigene: 220224 Mouse

Unigene: 91361 Rat



Picture

Sample: HepG2 Cell Lysate at 30 ug
Primary: Anti-GluR2 (SL1798R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 117 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (SL1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (SL1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated(SL1798R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ugl in 100 uL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue) Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
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